y10b antibody (Santa Cruz Biotechnology)
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Y10b Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/y10b+antibody/rRNA+Antibody/bio_rxiv__2024__12__22__629968-209-7-9
Average 92 stars, based on 24 article reviews
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Lysis:Article Title: Cytosolic Ptbp2 modulates axon growth in motoneurons through axonal localization and translation of Hnrnpr Article Snippet: .. The pellet was lysed in 700 μl of lysis buffer (300 mM KCl, 2 mM MgCl 2 , 20 mM Tris-HCl pH 7.4, 2 mM DTT, 0.05% sodium deoxycholate, 100 μg/ml cycloheximide) for 20 min on ice and cleared by centrifugation at 20,000 × g , 4 °C for 10 min. 1 μg of Article Title: Cytosolic Ptbp2 modulates axon growth in motoneurons through axonal localization and translation of Hnrnpr. Article Snippet: .. 1 μg of Article Title: hnRNP R promotes O-GlcNAcylation of eIF4G and facilitates axonal protein synthesis Article Snippet: .. The lysate was incubated for 20 min on ice and cleared by centrifugation at 20,000 × g , 4 °C for 10 min followed by incubation with RNase I (7.5 U per unit absorbance at 260 nm of lysate) at room temperature for 45 min. 10 μl of magnetic Dynabeads Protein G were bound to 1 μg Centrifugation:Article Title: Cytosolic Ptbp2 modulates axon growth in motoneurons through axonal localization and translation of Hnrnpr Article Snippet: .. The pellet was lysed in 700 μl of lysis buffer (300 mM KCl, 2 mM MgCl 2 , 20 mM Tris-HCl pH 7.4, 2 mM DTT, 0.05% sodium deoxycholate, 100 μg/ml cycloheximide) for 20 min on ice and cleared by centrifugation at 20,000 × g , 4 °C for 10 min. 1 μg of Article Title: hnRNP R promotes O-GlcNAcylation of eIF4G and facilitates axonal protein synthesis Article Snippet: .. The lysate was incubated for 20 min on ice and cleared by centrifugation at 20,000 × g , 4 °C for 10 min followed by incubation with RNase I (7.5 U per unit absorbance at 260 nm of lysate) at room temperature for 45 min. 10 μl of magnetic Dynabeads Protein G were bound to 1 μg Incubation:Article Title: Cytosolic Ptbp2 modulates axon growth in motoneurons through axonal localization and translation of Hnrnpr Article Snippet: .. The pellet was lysed in 700 μl of lysis buffer (300 mM KCl, 2 mM MgCl 2 , 20 mM Tris-HCl pH 7.4, 2 mM DTT, 0.05% sodium deoxycholate, 100 μg/ml cycloheximide) for 20 min on ice and cleared by centrifugation at 20,000 × g , 4 °C for 10 min. 1 μg of Article Title: hnRNP R promotes O-GlcNAcylation of eIF4G and facilitates axonal protein synthesis Article Snippet: .. The lysate was incubated for 20 min on ice and cleared by centrifugation at 20,000 × g , 4 °C for 10 min followed by incubation with RNase I (7.5 U per unit absorbance at 260 nm of lysate) at room temperature for 45 min. 10 μl of magnetic Dynabeads Protein G were bound to 1 μg Control:Article Title: Cortical projection neurons with distinct axonal connectivity employ ribosomal complexes with distinct protein compositions Article Snippet: .. We used one tube for pulldown with Article Title: hnRNP R promotes O-GlcNAcylation of eIF4G and facilitates axonal protein synthesis Article Snippet: .. The lysate was incubated for 20 min on ice and cleared by centrifugation at 20,000 × g , 4 °C for 10 min followed by incubation with RNase I (7.5 U per unit absorbance at 260 nm of lysate) at room temperature for 45 min. 10 μl of magnetic Dynabeads Protein G were bound to 1 μg |


